![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K2014000:Design
Designed by: Przemyslaw Nuc Group: iGEM16_UAM_Poznan (2016-10-11)
pBAD-E15'UTR->sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 236
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 53
Illegal SapI.rc site found at 365
Design Notes
One of our goals was to find the optimal or the best performing and universal 5'UTR for several E. coli derived inducible promoters in our multipromoter expression system.
Source
pBAD promoter is a fragment of Eschericha coli genome; 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).