Generator

Part:BBa_K2014000:Design

Designed by: Przemyslaw Nuc   Group: iGEM16_UAM_Poznan   (2016-10-11)


pBAD-E15'UTR->sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 53
    Illegal SapI.rc site found at 365


Design Notes

One of our goals was to find the optimal or the best performing and universal 5'UTR for several E. coli derived inducible promoters in our multipromoter expression system.


Source

pBAD promoter is a fragment of Eschericha coli genome; 5'UTR is synthetic, similar to a transcript of T7 bacteriophage,sfGFP is synthetic, based on the publication of Jean-Denis Pedelacq et all (2006).

References